MAXgene GMP Transfection Reagent is a cGMP transfection reagent for the development and manufacture of viral vectors for cell and gene-based therapies. It is an ideal reagent in the HEK293 and CHO systems for the manufacture of AAV, LV, and recombinant proteins. MAXgene® GMP leverages the efficiency and scalability of Polysciences’ PEI MAX® while adding the validation process and regulatory components necessary to move to clinical and commercial manufacturing. It is manufactured in accordance with cGMP under an ISO 13485 Quality Management System.
- Compatible with different virus production platforms.
- High transfection efficiency
- Reliable and scalable performance
- cGMP and ISO 13485
- Validated manufacturing processes
- Totally synthetic, free of animal origin.
Shipping Requirements: a cold pack
Storage: Store at 2-8 degrees Celsius.
X-tremeGENE siRNA Transfection Reagent efficiently delivers short interfering RNA (siRNA) into many commonly used cell types, including HeLa, NIH 3T3, HEK-293, CHO-K1, and COS-7, and various lines difficult-to-transfect cells, such as HT29, a human adenocarcinoma cell line.
Features and Benefits
- It suppresses gene expression by over 90% in many different cell types.
- Maximize experimental flexibility with a single reagent for co-transfection and siRNA-based gene knockout experiments.
- Produce meaningful results by using a reagent that exhibits low cytotoxicity, ensuring that the cellular effects you observe are due to the transfected siRNA rather than the transfection procedure.
- Work with or without serum, avoiding medium changes (eg, to serum-free medium) before or after transfection;
X-tremeGENE siRNA Transfection Reagent is a proprietary blend of lipids and other components, free of animal products.
Activity Assay: X-tremeGENE siRNA Transfection Reagent (1 – 2.5 µl) is combined with siRNA (0.1 – 0.35 µg) that is specific for the HPRT housekeeping gene. The mixture is used to transfect HEK-293 cells (in a monolayer, 30-50% confluent) in the presence of 10% fetal bovine serum (FBS). After transfection, the cells’ decline in HPRT mRNA is determined with the LightCycler® real-time PCR system. A reduction efficiency of 70 to 95% is typically observed when measuring mRNA.
Cytotoxicity analysis: HEK-293 cells are exposed to siRNA/X-tremeGENE siRNA reagent complexes for 72 hours in the presence of serum, without medium change. Cytotoxicity is then tested by assaying cells with WST-1 cell proliferation reagent (Roche).